Scientific Publications

Title:: In vitro study of a novel polymeric mesenchymal stem-cell coated membrane

Authors: Ada Grin, Yoel Sasson, Shaul Beyth, Rami Mosheiff, Jacob Rachmilewitz, Michael Friedman

Journal: Journal of Drug Delivery Science Technology 19 (4) (2009): 241-246.

Abstract: The objective of the study was to develop and study in vitro polymeric membrane that enables adhesion, proliferation, and differentiation of mesenchymal stem cells on its surface. We screened several membrane formulations with varying plasticizer types and concentrations for their ability to support MSC adherence and growth. To that end, MSCs were isolated from human bone marrow, and cell adherence to membrane, cell membrane interaction and cell morphology were observed using scanning electron microscopy (SEM) and fluorescent microscopy. MSCs adhered well to Ammonio Methacrylate copolymer type A (AMCA) membranes prepared with the various plasticizers and revealed spindle monolayer shape with podia, similar to polystyrene dishes. MSCs proliferated and differentiated only on AMCA membrane with polyethyleneglycol 400 (PEG400) as plasticizer. Furthermore, the membrane surfaces were characterized using SEM and their porosity was measured. These data confirm that MSCs maintain their stem cell traits after adhesion to membrane.

Title:: Quantification of guided regeneration of weight-bearing bones

Authors: Rami Mosheiff, Adi Friedman, Michael Friedman, Meir Liebergall

Journal: Orthopedics 26 (8) (August 2003):  789-794.

Abstract: An accurate histological evaluation system was established to improve and quantify the compatibility of membranes to provide bone regeneration in a large mid-diaphyseal bone defect, complementing radiographic evaluation. New bone formation obtained in critical segmental defects covered with tubular ethyl cellulose membranes was examined. Computerized radiography showed gradual new bone formation, which slows after 6 weeks. In a parallel histomorphological assessment, a characteristic process of new bone formation occurs inside the confined space and surrounding tissues. Histological findings are summarized according to the new scoring system, which clarifies the mechanism and provides complementary quantification of guided bone regeneration.
 

Technology

AMCA Bone Membrane

REGENECURE's regenerative biocompatible polymeric membrane implants are made of microporous AMCA (ammonio methacrylate copolymer type A) and a plasticizer.

The microporous surface of the membrane facilitates the adherence of stem cells (MSCs) recruited to the injured site through a signaling mechanism known as chemotaxis. The membrane allows excellent proliferation and differentiation of MSCs into the bone tissue. The membrane implant is strong and flexible, and serves as an effective barrier to prevent connective tissue from infiltrating the healing space and slowing down the regenerative healing process.

REGENECURE’s implant can be shaped into a tube or a patch to span large segmental bone defects. The osteoconductive and osteoinductive qualities of the membrane ensure that REGENECURE’s MSC scaffolding method directs bone in-growth naturally into the tubular implant.

The membrane implant operates both as a delivery system for cells and as a device for guided bone regeneration or tissue engineering purposes. It can also be used to deliver active agents such as osteoinductive, antiseptic agents, and more.

Preclinical results have shown excellent healing, including complete restoration of bone, eight weeks post-osteotomy in a critical size defect model.


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